Apo Idanwo Apapọ SARS-COV-2 (Elisa)

Apo Idanwo Apapọ SARS-CoV-2 (ELISA) da lori idanwo ajẹsara ajẹsara fun ipinnu ti awọn ọlọjẹ SARS-CoV-2 ninu omi ara eniyan, pilasima (EDTA, Heparin tabi iṣuu soda citrate).Awọn kanga awo micro bi ipele ti o lagbara ni a bo pẹlu atunmọ SARS-CoV-2 recombinant Receptor Binding Domain protein.Ni igbesẹ idawọle akọkọ ti o baamu awọn aporo-ara kan pato (SARS-CoV-2-IgG-Ab & diẹ ninu awọn IgM-Ab) ti o wa ninu awọn apẹẹrẹ alaisan sopọ mọ awọn antigens ni ipele to lagbara.Ni opin abeabo unbound irinše ti wa ni fo jade.Fun igbesẹ abeabo keji SARS-CoV-2 recombinant Olugba abuda ase amuaradagba conjugate (SARS-CoV-2 recombinant Receptor Binding Domain protein peroxidase conjugate) ti wa ni afikun eyiti o sopọ ni pataki si awọn ọlọjẹ SARS-CoV-2 (pẹlu IgG ati IgM) ti o yọrisi awọn Ibiyi ti aṣoju immunocomplexes.Lẹhin igbesẹ fifọ keji lati yọkuro conjugate apọju, TMB/Substrate ti wa ni afikun (igbesẹ3).Awọ buluu kan ndagba iyipada si ofeefee lẹhin didaduro iṣesi pẹlu ojutu iduro.Gbigba ti awọn calibrators ati apẹrẹ jẹ ipinnu nipasẹ lilo oluka awo micro ELISA.Awọn abajade fun awọn ayẹwo alaisan ni a gba nipasẹ lafiwe pẹlu iye gige kan.